DNase I

Proveedor: AGILENT

600031 600032
AGLS600031EA 197 EUR
AGLS600031 AGLS600032
DNase I
Enzimas

Purified DNase I tested to be free of contaminating activity for the degradation of nucleic acids.


  • RNase free
  • Contains a mixture of four isoenzymes from bovine pancreas (10 units/µl)


DNase I, RNase Free catalyses the degradation of double-stranded DNA into oligonucleotides 1,2 and mononucleotides. This enzyme has been isolated as a mixture of four isoenzymes from bovine pancreas that cut preferentially next to pyrimidine nucleotides.


Reaction Conditions: 40 mM Tris-HCl (pH 7,5), 6 mM MgCl₂, 2 mM CaCl₂, 1 µg DNA and 1 unit enzyme in a 25 µl volume. Incubate 30 minutes at 37 °C.


Precaución: For research use only. Not for use in diagnostic procedures.

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ESPECIFICACIONES

Nombre de la enzima DNase I
Environmentally Preferable
Fuente
Especies
Forma Premixed
Formulación 10 mM Tris-HCl (pH 7,5), 10 mM CaCl₂, 10 mM MgCl₂ and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)

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