2',7'-Dichlorofluorescin diacetate ≥95% (by HPLC)

Proveedor: ENZO LIFE SCIENCES
Atención

Sinónimos: H2DCFDA, 2,7-Dichlorodihydrofluorescein diacetate, DCDHF diacetate

ALX610022M050
ENZOALX610022M050EA 69.3 EUR
ENZOALX610022M050
2',7'-Dichlorofluorescin diacetate ≥95% (by HPLC)
2',7'-Dichlorofluorescin diacetate

Peroxynitrite indicator.


Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalysed cleavage of the diacetate groups, DCDHF is oxidised by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502 nm, Em(max): 523 nm), or by absorbance spectroscopy at 500 nm (ε = 59, 500 M⁻¹cm⁻¹). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidise DCDHF.


25 mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide, sparingly soluble in aqueous buffers.

Formula: C₂₄H₁₆Cl₂O₇
Temperatura de almacenaje: Congelador
Número MDL: MFCD00128955
Núm. CAS: 4091-99-0

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Resultados test especificaciones

Purity ≥95% (HPLC)


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