CoV Protease Substrate-1 EDANS

Proveedor: STEMCELL Technologies

100-0507
STMC100-0507EA 398 EUR
STMC100-0507
CoV Protease Substrate-1 EDANS
Proteínas y Péptidos

FRET-based peptide substrate for detection of protease activity of coronaviruses - 100 tests.


  • Enables efficient detection of coronavirus protease activity
  • Can be used as a high throughput screening tool for inhibitors of coronavirus proteases


CoV Protease Substrate-1 EDANS allows for efficient detection of coronavirus (CoV) proteases. This fluorescence resonance energy transfer (FRET)-based peptide substrate can be cleaved by 3C-like protease (3CLPro), the CoV main protease involved in regulating the coronavirus replication complex. 3CLPro is also a potential target for therapeutic applications.


CoV Protease Substrate-1 EDANS has an excitation wavelength of 336 nm, an emission wavelength of 455 nm, and an extinction coefficient of 5900 cm⁻¹ M⁻¹. It consists of a 14 amino acid sequence (KTSAVLQSGFRKME) that also contains the DABCYL (quencher) and EDANS (donor) fragments at the N- and C- termini, respectively. When the peptide substrate is intact, favorable energetic overlap of the EDANS excited state and the DABCYL absorption leads to efficient intramolecular fluorescence quenching of EDANS by the DABCYL fragment. Cleavage of the substrate by CoV proteases, however, dissociates the EDANS fragment from DABCYL, restoring the fluorescence of the EDANS moiety that is directly correlated to the CoV protease activity.


Precaución: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').

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