Esta buscando: Biochemistry Learning Activities

Numero del catalogo: (BE-404)

Proveedor: G-Biosciences

Descripción: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

UOM: 1 * 1 KIT

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Numero del catalogo: (BE-401)

Proveedor: G-Biosciences

Descripción: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO300)

Proveedor: EDVOTEK

Descripción: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO282)

Proveedor: EDVOTEK

Descripción: This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO202)

Proveedor: EDVOTEK

Descripción: Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO212)

Proveedor: EDVOTEK

Descripción: El ADN del bacteriófago Lambda es una molécula lineal bien caracterizada que contiene seis sitios de reconocimiento para <i>Eco</i> RI (5 sitios distintos; 2 de ellos de tamaño muy similar). En este experimento, el ADN de Lambda es digerido por la endonucleasa <i>Eco</i> RI. Los productos de la digestión se analizan mediante electroforesis en gel de agarosa.

UOM: 1 * 1 KIT

Promoción

Proveedor: EDVOTEK

Descripción: The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Numero del catalogo: (EDVO225)

Proveedor: EDVOTEK

Descripción: This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.

UOM: 1 * 1 KIT

FDS Promoción

Numero del catalogo: (EDVO266)

Proveedor: EDVOTEK

Descripción: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.

UOM: 1 * 1 KIT

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Numero del catalogo: (EDVOS-47)

Proveedor: EDVOTEK

Descripción: Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO303)

Proveedor: EDVOTEK

Descripción: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO235)

Proveedor: EDVOTEK

Descripción: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO203)

Proveedor: EDVOTEK

Descripción: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (EDVO302)

Proveedor: EDVOTEK

Descripción: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (BE-408)

Proveedor: G-Biosciences

Descripción: En esta actividad de laboratorio, los estudiantes aprenden a realizar procedimientos simples de aislamiento de proteínas para aislar la huella proteica de varios tejidos frescos. Compararán las huellas proteicas de 4 tejidos diferentes para comprender que la función de un órgano en particular se debe a las proteínas que se localizan en el órgano específico. Este kit también incluye cuatro muestras de proteínas secas (de hígado, cerebro, corazón y pulmones de ratón) para comparar como control, si no se pueden obtener tejidos frescos.

UOM: 1 * 1 KIT

Promoción

Numero del catalogo: (BE-407)

Proveedor: G-Biosciences

Descripción: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

UOM: 1 * 1 KIT

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