Human HRV 3C protease solution kit
Proveedor: Thermo Fisher Scientific
The Pierce™ Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
- Highly specific with no off-target cleavage even at high enzyme/substrate concentration
- Allows cleavage reaction to take place at 4°C without affecting the functionality of purified protein
- Can be used either following affinity purification or while fusion proteins are bound to glutathione or a tag-specific resin
- Dual-tagged enzyme is compatible with various columns and buffers
The HRV 3C protease enzyme (47,8 kDa) is genetically engineered and contains both GST and 6×His tags so that it can be removed by Ni, cobalt or glutathione (GSH) resin. It is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. This is a restriction grade protease that has robust activity at 4 °C.
Información de suministro: The kit contains HRV 3C protease (2 units/µl), reaction buffer and GST-Syk fusion protein.
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