Pierce™ Glutathione chromatography cartridges are convenient, ready to use pre-packed devices for the purification of GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.
- Binds at least 40 mg of purified recombinant GST protein per ml of resin
- Consistently purifies at least 10 mg of GST-tagged protein per ml of resin with greater than 90% purity
- Resin can be reused at least five times without reduction in binding capacity and purification performance
- Works well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions
Pierce™ Glutathione chromatography cartridges are compatible with the major automated liquid-chromatography systems or manual syringe processing. The cartridges attach directly to ÄKTA™ or FPLC Systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-Lok® syringe fittings or 1/16" tubing. These cartridges enable fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.
Información de suministro: Kit includes an accessory pack (1 female Luer-Lok® adapter, 1 connector fitting, 1 column plug and bottom caps).
