o-Nitrofenil-B-D-galactopiranósido (ONPG), crystalline
Proveedor: MP Biomedicals
Sinónimos:
o-Nitrophenyl-β-D-galactoside, 2-Nitrophenyl β-D-Galactopyranoside, O-Nitrophenyl-Β-D-Galactopyranoside, ONPG, (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol
o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
Soluble in water (10 mg/ml- clear, colorless solution; 15 mg/ml - clear, faint yellow solution; 20 mg/ml with heat - clear, yellowish solution).
Precaución: Research Use Only (RUO).
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Formula:
C₁₂H₁₅NO₈ MW: 301,25 g/mol Temperatura de almacenaje: Nevera |
Número MDL:
MFCD00063255 Núm. CAS: 369-07-3 |
Resultados test especificaciones
| Identity test | Passes |
| FTIR | Conforms to standard |
| Optical Rotation (c=1, H₂O) | –66° ± 7° |
| Melting Point | 180 - 195 °C |
| UV/VIS: λmax (H₂O) | 262 ±3 nm |
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