Violeta Cristal ethanolic aqueous solution primary stain for Gram staining, Sigma-Aldrich®
Proveedor: Merck
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Atención
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Sinónimos:
Hexametil-p-rosanilina cloruro, 4-[4,4'-Bis(dimetilamino)bencihidrolideno]cicloexa-2,5-dieno-1-ilideno] dimetilamonio cloruro hexametilpararrosanilina cloruro, CI 42555, Violeta básico 3
The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.
The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Formula:
C₂₅H₃₀ClN₃ MW: 407.99 g/mol Temperatura de almacenaje: Ambiente |
Número MDL:
MFCD00011750 Núm. CAS: 548-62-9 UN: 1170 ADR: 3 III Índice Merck: 13,04406 |
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