Human Recombinant TNF-A (Animal free) (from E. coli)

Proveedor: Shenandoah Biotechnology

100-111AF-100U 100-111AF-10UG 100-111AF-1MG 100-111AF-500U 100-111AF-50UG
SHBT100-111AF-100UEA 356 EUR
SHBT100-111AF-100U SHBT100-111AF-10UG SHBT100-111AF-1MG SHBT100-111AF-500U SHBT100-111AF-50UG
Human Recombinant TNF-A (Animal free) (from E. coli)
Proteínas y Péptidos

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS).


  • High quality
  • Low endotoxin
  • Affordable


TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.


Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.


Información de suministro: A certificate of analysis (COA) is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions.


Precaución: For research use only.

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ESPECIFICACIONES

Nombre de proteína / péptido TNF-alpha TNF-alpha
Environmentally Preferable
Sinónimos de proteínas Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin DIF|cachexin|cachectin|necrosin|TNFSF2|TNF|cytotoxin
Tipo de proteína / péptido Recombinant Recombinant
Función de la proteína Cytokine Cytokine
Especies Human Human
Fuente E. coli E. coli
Conjugado Unconjugated
ID del gen P01375
Sequence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Sin proteasa N
Libre de animales Y Y
Sin nucleasas N
Sin transportista Y
Sin endotoxina N
Nivel de endotoxina ≤1 EUs/μg Low
Contenido de endotoxinas Endotoxin LAL (EU/µg) ≤1
UniProtKB P01375 P01375
Actividad biológica Cytolysis of mouse L929 cells in the presence of actinomycin D at ≤2 ng/ml (or5×10⁵ units/mg) Bioactivity assay: Cytolysis of mouse L929 cells in the presence of actinomycin D, ED50 ≤2 ng/ml, bioactivity ≥5,0×10⁵ units/mg
Pureza >95% ≥95%
Grado RUO
Formulación Lyophilised from a sterile (0,2 µm) filtered aqueous solution containing 10 mM sodium phosphate, pH 7,5 10 mM sodium phosphate, pH 7,5
Molecular weight 17,5 kDa (monomer) 17,5 kDa
Instrucciones de reconstitución Reconstitute with sterile water at 0,1 mg/ml Sterile water at 0,1 mg/ml
Condiciones de almacenamiento −20 °C −20 °C
Shipping temperature Ambient RT
Tested applications Bioassay Bioassay

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