Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R), Sigma-Aldrich®
Proveedor: SIGMA-ALDRICH MICROSCOPY
M-Clarity™ quality level = MQ200
Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.
- TRITC is used to label a wide variety of biomolecules, including immunoglobulins, lectins, nucleic acids, polynucleotides, and polysaccharides for affinity, immuno-, and in situ hybridization, fluorescent probes, and flow cytometry
- Lectins are TRITC-Iabeled reagents for the affinity staining of sections and cell monolayers to distinguish the sialomucins of salivary glands
- TRITC-antibodies are used to identify pathogenic amoebae
- TRITC-oligonucleotides are used in the in-situ hybridization staining of soil microorganisms
- TRITC-Iabeled reagents have also been used as fluorescent probes of live cells
- Flow cytometry of TRlTC-Iabeled slime mold cells has been used to study their aggregation behavior
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Formula:
C₂₅H₂₁N₃O₃S Temperatura de almacenaje: Nevera |
Número MDL:
MFCD00063517 Núm. CAS: 80724-20-5 |
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Resultados test especificaciones
| Appearance (Form) | Powder |
| Solubility (Colour) | Faint Red to Very Dark Red to Very Dark Purple |
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Solubility (Form) 10 mg/mL , 1M NH4OH in MeOH |
Solution |
| 1H NMR Spectrum | Conforms to Structure |
| Solvent Content (by GC) | ≤4 % |
| Nitrogen | ≥ 7.5 % |
| Dye Content | ≥80 % |
| Labeling Efficiency | ≥50 % |
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