Azul nitro-tetrazolio cloruro (NBT), yellow powder
Proveedor: MP Biomedicals
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Peligro
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Sinónimos:
Dicloruro de 5,5'-difenil-3,3'-bis(4-nitrofenil)-2,2'-(3,3'-dimetoxibifenil-4,4'-ilen)ditetrazolio
p-Nitroblue Tetrazolium Chloride is a substrate for dehydrogenases and other oxidases and precipitating agent for fibrinogen.
It is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
It is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Slightly (10 mg/ml) soluble in water.
Formula:
C₄₀H₃₀Cl₂N₁₀O₆ MW: 817,65 g/mol Pto. de fusión: 189…192 °C (lit.) Temperatura de almacenaje: Nevera |
Número MDL:
MFCD00012159 Núm. CAS: 298-83-9 EINECS: 206-067-4 |
Resultados test especificaciones
identity Test | Passes |
l max (MeOH) | 255 ± 3 nm |
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