Ácido 5-fluoroorotico enzyme substrate

Proveedor: Thermo Fisher Scientific

R0811 R0812
R0811EA 99.75 EUR
R0811 R0812
Ácido 5-fluoroorotico enzyme substrate
Ácido 5-fluoroorotico

Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase


Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).


Yeasts with an active URA3 gene (Ura+) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells

Formula: C₅H₃FN₂O₄
Número MDL: MFCD00042526
Núm. CAS: 703-95-7
EINECS: 211-876-0

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