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Esta buscando: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER16180

Pierce™ Firefly Signal Enhancer (100X)

Numero del catalogo: (PIER16180)
Proveedor: Thermo Fisher Scientific
Descripción: Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.
UOM: 1 * 0,5 mL
Product Image-PIER32110

Antibody extender solution NC, Miser™

Numero del catalogo: (PIER32110)
Proveedor: Thermo Fisher Scientific
Descripción: Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.
UOM: 1 * 500 mL

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Product Image-786-123T

Sample clean up, PAGE-Perfect™

Proveedor: G-Biosciences
Descripción: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Image Unavailable-69078-3

Western blotting kit, Perfect Protein™, Novagen®

Numero del catalogo: (69078-3)
Proveedor: Merck Millipore (Novagen)
Descripción: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.
UOM: 1 * 1 UN
Product Image-ROCK88-8884-31

Western blot kits, TrueBlot®

Proveedor: Rockland Immunochemicals
Descripción: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

FDS

Product Image-ROCKFEMTOMAX-020

Chemiluminescent super sensitive HRP substrate, FemtoMax™

Proveedor: Rockland Immunochemicals
Descripción: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Image Unavailable-J60561.AP

SSC (20X), RNAse free

Proveedor: Thermo Fisher Scientific
Descripción: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

FDS Certificados

Product Image-PIER35065

Fast western blotting kit, SuperSignal™ west Pico, Pierce™

Proveedor: Thermo Fisher Scientific
Descripción: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Image Unavailable-WB59-100

Western-Re-Probe™ reagent, Calbiochem®

Numero del catalogo: (WB59-100)
Proveedor: Merck Millipore (Oncogene)
Descripción: This Western-Re-Probe™ reagent is validated for use in immunoblotting.
UOM: 1 * 100 mL
Image Unavailable-J63209.AK

Saponin permeating solution, 0,5% w/v solution in PBS (10X)

Numero del catalogo: (J63209.AK)
Proveedor: Thermo Fisher Scientific
Descripción: Saponin permeating solution, is used as a cell permeabilising agent.
UOM: 1 * 250 mL

FDS Certificados


Product Image-PIER35081

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Proveedor: Thermo Fisher Scientific
Descripción: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Product Image-786-124T

Sample clean up, Perfect-FOCUS™

Proveedor: G-Biosciences
Descripción: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Image Unavailable-J61462.AK

Luminol and oxidising solutions

Proveedor: Thermo Fisher Scientific
Descripción: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

FDS Certificados

Product Image-PIER15159

Enhanced chemifluorescent HRP substrate kit, QuantaRed™

Numero del catalogo: (PIER15159)
Proveedor: Thermo Fisher Scientific
Descripción: QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.
UOM: 1 * 1 UN
Product Image-TB110

Kingdom blot

Numero del catalogo: (TB110)
Proveedor: G-Biosciences
Descripción: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM: 1 * 1 UN
Product Image-ROCKKCA001

Chemiluminescent western blot kits, FemtoMax™

Proveedor: Rockland Immunochemicals
Descripción: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

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El stock para este material es limitada pero puede estar disponible en un almacén cerca de usted. Por favor, asegúrese de que ha iniciado sesión en la web para que el stock disponible se puede mostrar. Si el call sigue apareciendo y usted necesita ayuda, por favor llámenos al 902 222 897 o por email en webshop.es@avantorsciences.com.
El stock para este material es limitada pero puede estar disponible en un almacén cerca de usted. Por favor, asegúrese de que ha iniciado sesión en la web para que el stock disponible se puede mostrar. Si el call sigue apareciendo y usted necesita ayuda, por favor llámenos al 902 222 897 o por email en webshop.es@avantorsciences.com.
Este producto se trata de un artículo regulado sometido a normativa que restringe su venta. Si procede, nos pondremos en contacto con usted para solicitarle la licencia o declaración de uso necesaria para poder proceder al suministro del producto.
Este producto se trata de un artículo regulado sometido a normativa que restringe su venta.
Si procede, nos pondremos en contacto con usted para solicitarle la licencia o declaración de uso necesaria para poder proceder al suministro del producto.
Este producto ha sido bloqueado por su organización. Por favor, pónganse en contacto con su departamento de compras para obtener más información.
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