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Esta buscando: Reactivos para extracción de proteinas

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-786-244

SilverOUT™, G-Biosciences

Numero del catalogo: (786-244)
Proveedor: G-Biosciences
Descripción: SilverOUT removes interfering silver ions from gel pieces.
UOM: 1 * 100 Tests
Image Unavailable-ENZOADI9502730500

Antigen retrieval reagent, IHC

Proveedor: ENZO LIFE SCIENCES
Descripción: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

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Product Image-786-793

Immobilised Ficin

Numero del catalogo: (786-793)
Proveedor: G-Biosciences
Descripción: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
UOM: 1 * 5 mL
Product Image-786-681

InGel™ Blue for protein digestion

Numero del catalogo: (786-681)
Proveedor: G-Biosciences
Descripción: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 KIT
Product Image-DGA01

Optimizer blueBALLS™

Numero del catalogo: (DGA01)
Proveedor: G-Biosciences
Descripción: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 UN
Product Image-786-598

Biotin resin

Numero del catalogo: (786-598)
Proveedor: G-Biosciences
Descripción: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM: 1 * 5 mL
Product Image-786-241

InGel™ for protein digestion

Numero del catalogo: (786-241)
Proveedor: G-Biosciences
Descripción: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
UOM: 1 * 1 KIT
Product Image-786-077

Protein reducing agents

Proveedor: G-Biosciences
Descripción: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Product Image-786-599

Immobilised Iminobiotin Resin

Numero del catalogo: (786-599)
Proveedor: G-Biosciences
Descripción: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierce™

Numero del catalogo: (PIER90054)
Proveedor: Thermo Fisher Scientific
Descripción: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 50 µG

MSDS


Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™

Numero del catalogo: (PIER90056)
Proveedor: Thermo Fisher Scientific
Descripción: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 100 µG
Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierce™

Numero del catalogo: (PIER90053)
Proveedor: Thermo Fisher Scientific
Descripción: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 2 µG

MSDS


Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Numero del catalogo: (ENZOBMLKI1761250)
Proveedor: ENZO LIFE SCIENCES
Descripción: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL

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Product Image-PIER88701

Universal nuclease for cell lysis, Pierce™

Proveedor: Thermo Fisher Scientific
Descripción: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Image Unavailable-PIER90067

Protein quantitation reagent, TMTzero™ label reagent

Numero del catalogo: (PIER90067)
Proveedor: Thermo Fisher Scientific
Descripción: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLKI1050300

Fluor-de-Lys® Developer I concentrate

Numero del catalogo: (ENZOBMLKI1050300)
Proveedor: ENZO LIFE SCIENCES
Descripción: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl

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El stock para este material es limitada pero puede estar disponible en un almacén cerca de usted. Por favor, asegúrese de que ha iniciado sesión en la web para que el stock disponible se puede mostrar. Si el call sigue apareciendo y usted necesita ayuda, por favor llámenos al 902 222 897 o por email en webshop.es@avantorsciences.com.
El stock para este material es limitada pero puede estar disponible en un almacén cerca de usted. Por favor, asegúrese de que ha iniciado sesión en la web para que el stock disponible se puede mostrar. Si el call sigue apareciendo y usted necesita ayuda, por favor llámenos al 902 222 897 o por email en webshop.es@avantorsciences.com.
Este producto se trata de un artículo regulado sometido a normativa que restringe su venta. Si procede, nos pondremos en contacto con usted para solicitarle la licencia o declaración de uso necesaria para poder proceder al suministro del producto.
Este producto se trata de un artículo regulado sometido a normativa que restringe su venta.
Si procede, nos pondremos en contacto con usted para solicitarle la licencia o declaración de uso necesaria para poder proceder al suministro del producto.
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