Esta buscando: Instrumentos+Microarray

Numero del catalogo: (BOSSBS-1135R-HRP)

Proveedor: Bioss

Descripción: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

Promoción

Numero del catalogo: (BOSSBS-1135R-A555)

Proveedor: Bioss

Descripción: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

Promoción

Numero del catalogo: (BOSSBS-1135R-CY7)

Proveedor: Bioss

Descripción: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

Promoción

Numero del catalogo: (BOSSBS-1135R-FITC)

Proveedor: Bioss

Descripción: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

Promoción

Numero del catalogo: (RPK0325)

Proveedor: Cytiva

Descripción: Microarray hybridisation solution version 2.0, for highly reproducible microarray results.

UOM: 1 * 1 UN

Promoción

Numero del catalogo: (EDVO235)

Proveedor: EDVOTEK

Descripción: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

UOM: 1 * 1 KIT

Promoción

Proveedor: Greiner Bio-One

Descripción: Plastic plates for high-throughput microarrays. With 96 shallow wells and a low well rim, the HTA™ Plate has been optimised for the simultaneous analysis of multiple samples.

Numero del catalogo: (ANTIA84635-100)

Proveedor: ANTIBODIES.COM

Descripción: Goat polyclonal antibody to CTAG1B for ELISA, IF, flow cytometry and Microarray with samples derived from human.

UOM: 1 * 100 µG

New Product Promoción

Proveedor: Thermo Fisher Scientific

Descripción: Microarray slides for <i>in situ</i> hybridisation and amplification with standard microscope slides.

Numero del catalogo: (ANTIA304801-100)

Proveedor: ANTIBODIES.COM

Descripción: Mouse monoclonal [N68/6] antibody to Nav1.7 for WB, IHC, ICC/IF, IP and antibody microarray with samples derived from human, mouse, rat and hamster.

UOM: 1 * 100 µG

New Product Promoción

Numero del catalogo: (ANTIA304815-100)

Proveedor: ANTIBODIES.COM

Descripción: Mouse monoclonal [N22/21] antibody to SHANK1 for WB, IHC and antibody microarray with samples derived from human, mouse and rat.

UOM: 1 * 100 µG

New Product Promoción

Numero del catalogo: (ANTIA305021-100)

Proveedor: ANTIBODIES.COM

Descripción: Mouse monoclonal [N43/6] antibody to KCNQ4 for WB, IHC, ICC/IF, IP and antibody microarray with samples derived from human, mouse and rat.

UOM: 1 * 100 µG

New Product Promoción

Numero del catalogo: (ANTIA304814-100)

Proveedor: ANTIBODIES.COM

Descripción: Mouse monoclonal [N23b/6] antibody to SHANK2 for WB, IHC, IP, ICC/IF and antibody microarray with samples derived from human, mouse and rat.

UOM: 1 * 100 µG

New Product Promoción

Numero del catalogo: (ANTIA304805-100)

Proveedor: ANTIBODIES.COM

Descripción: Mouse monoclonal [Tsp232A] antibody to TSP23 for WB, IHC and antibody microarray with samples derived from human and mouse.

UOM: 1 * 100 µG

New Product Promoción

Numero del catalogo: (ANTIA304793-100)

Proveedor: ANTIBODIES.COM

Descripción: Mouse monoclonal [N1664A] antibody to DMPO for WB, IHC, ICC/IF, IP, ELISA and antibody microarray with samples derived from species independent.

UOM: 1 * 100 µG

New Product Promoción

Numero del catalogo: (ANTIA304821-100)

Proveedor: ANTIBODIES.COM

Descripción: Mouse monoclonal [S74] antibody to TRPM7 for WB, IHC, ICC/IF and antibody microarray with samples derived from human, mouse and rat.

UOM: 1 * 100 µG

New Product Promoción