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Phire® Hot Start II DNA polymerase

Proveedor: FINNZYMES REAGENTS

Descripción: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

DyNAmo™ ColorFlash SYBR® Green qPCR Kits

Proveedor: FINNZYMES REAGENTS

Descripción: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Phusion® green hot start II high-fidelity PCR master mix

Proveedor: FINNZYMES REAGENTS

Descripción: Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Master mixes, green multiplex, Phusion® U

Proveedor: FINNZYMES REAGENTS

Descripción: Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Master mixes, hot start, Phusion® U

Proveedor: FINNZYMES REAGENTS

Descripción: Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

Phusion® High-Fidelity PCR Kit

Proveedor: FINNZYMES REAGENTS

Descripción: Phusion® High-Fidelity PCR Kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR Kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.

Phusion® Flash high-fidelity PCR master mix

Proveedor: FINNZYMES REAGENTS

Descripción: Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than <i>Taq</i>.