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Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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ReadiUse™ Bacterial Cell Lysis Buffer 5X (10 ml)

Numero del catalogo: (AATB24100)

Proveedor: AAT BIOQUEST

Descripción: Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.

UOM: 1 * 10 mL

Promoción

N/A Each

Subcellular protein fractionation kit for tissues

Proveedor: Thermo Fisher Scientific

Descripción: This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Protein extraction kit, Insect PE LB™

Numero del catalogo: (786-411)

Proveedor: G-Biosciences

Descripción: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

UOM: 1 * 250 mL

Promoción

N/A Each

Y-PER™ Yeast Protein Extraction Reagent

Proveedor: Thermo Fisher Scientific

Descripción: This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Tampones de extracción de ADN genómico

Proveedor: OMEGA BIO-TEK

Descripción: Tampón TL, Omega Bio-Tek

FDS

Extraction buffers, FOCUS™

Proveedor: G-Biosciences

Descripción: FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Nuclear and cytoplasmic extraction reagents, NE-PER

Proveedor: Thermo Fisher Scientific

Descripción: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Mammalian protein extract reagents, M-PER™

Proveedor: Thermo Fisher Scientific

Descripción: The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Proveedor: Merck Millipore (Novagen)

Descripción: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.