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Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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Reactivos para extracción de proteínas, BugBuster® y BugBuster® plus Benzonase®, Millipore®

Proveedor: Merck Millipore (Novagen)

Descripción: El reactivo para extracción de proteínas BugBuster® está formulado para romper suavemente la pared celular del <i>E. coli</i>, liberando proteína soluble. Proporciona una alternativa, sencilla, rápida y económica a métodos mecánicos como la french press o la sonicación, utilizados para la liberación de proteínas, como preparación para purificación posterior u otras aplicaciones. La formulación utiliza una mezcla de detergentes capaces de perforar la pared celular sin desnaturalizar la proteína soluble.

Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Numero del catalogo: (J61792.AP)

Proveedor: Thermo Fisher Scientific

Descripción: Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

UOM: 1 * 500 mL

FDS Certificados Promoción

N/A Each

NP-40 lysis buffer

Proveedor: Thermo Fisher Scientific

Descripción: NP-40 lysis buffer, Thermo Fisher Scientific

FDS Certificados

NP-40 lysis buffer with glycerol (2X)

Numero del catalogo: (J60143.AK)

Proveedor: Thermo Fisher Scientific

Descripción: NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

UOM: 1 * 250 mL

FDS Certificados Promoción

N/A Each

Triton® X-100 lysis buffer, pH 7,4

Proveedor: Thermo Fisher Scientific

Descripción: Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific

FDS Certificados

NP-40 lysis buffer, low salt

Proveedor: Thermo Fisher Scientific

Descripción: NP-40 lysis buffer, low salt, Thermo Fisher Scientific

FDS Certificados

MNE buffer, pH 6,5

Proveedor: Thermo Fisher Scientific

Descripción: MNE buffer, pH 6.5, Thermo Fisher Scientific

FDS Certificados

HEPES lysis buffer with NP-40 (2X)

Numero del catalogo: (J63867.AK)

Proveedor: Thermo Fisher Scientific

Descripción: HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

UOM: 1 * 250 mL

FDS Certificados Promoción

N/A Each

Protein extraction reagent, BugBuster® HT, Millipore®

Proveedor: Merck Millipore (Novagen)

Descripción: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.