This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.
- Potential of electrophoresis in protein research
- Use of electrophoresis for protein characterisation
- Parameters affecting protein electrophoresis analysis
- Analysis of proteins structure by electrophoresis
- Role of denaturing and reducing agents in protein electrophoresis
- Protein degradation, breakdown, and role of protease
Also required: Protein electrophoresis equipment, water bath or beaker and thermometer.
Información de suministro: Supplied with components needed for hands-on experimentation for six workstations of 4 to 5 students or 24 to 30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
