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Supelco® Organic Reference Standard, Acetonitrile

Numero del catalogo: (45983-5ML)

Proveedor: Merck

Descripción: Organic Standard, Acetonitrile, Tipo de env.: Botella de vidrio

UOM: 1 * 5 mL

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Supelco® Organic Reference Standard, 2-Methyltetrahydrofuran

Proveedor: Merck

Descripción: Organic Standard, 2-Methyltetrahydrofuran, Tipo de env.: Botella de vidrio

Azul nitro-tetrazolio cloruro (NBT) para biología molecular

Proveedor: MP Biomedicals

Descripción: p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.