Esta buscando: Merck Millipore (Novagen)

Proveedor: Merck Millipore (Novagen)

Descripción: El reactivo PopCulture® es un concentrado con base de detergente que se puede añadir directamente a cultivos de<i> E. coli </i>para extraer proteínas de forma efectiva sin necesidad de una recolección de células. Las proteínas recombinantes pueden estudiarse directamente en el extracto crudo o purificarse añadiendo una matriz de afinidad, lavando el complejo matriz-proteina dian para eliminar medio de cultivo y contaminantes celulares, y eluyendo la proteína purificada de la matriz. El procedimiento de purificación de proteínas PopCulture es ideal para el procesamiento robótico de muestras de alto rendimiento para la investigación en proteómica o cualquier aplicación que se beneficie de una rapidez y comodidad superior.

Proveedor: Merck Millipore (Novagen)

Descripción: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Proveedor: Merck Millipore (Novagen)

Descripción: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Numero del catalogo: (69909-3)

Proveedor: Merck Millipore (Novagen)

Descripción: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

UOM: 1 * 10 µG

Promoción

Proveedor: Merck Millipore (Novagen)

Descripción: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Numero del catalogo: (69750-3)

Proveedor: Merck Millipore (Novagen)

Descripción: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

UOM: 1 * 10 µG

Promoción

Proveedor: Merck Millipore (Novagen)

Descripción: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Numero del catalogo: (70527-3)

Proveedor: Merck Millipore (Novagen)

Descripción: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

UOM: 1 * 15 mL

Promoción

Numero del catalogo: (69704-4)

Proveedor: Merck Millipore (Novagen)

Descripción: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

UOM: 1 * 10 mL

Promoción

Proveedor: Merck Millipore (Novagen)

Descripción: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Proveedor: Merck Millipore (Novagen)

Descripción: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Proveedor: Merck Millipore (Novagen)

Descripción: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Numero del catalogo: (71281-3)

Proveedor: Merck Millipore (Novagen)

Descripción: ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

UOM: 1 * 1 UN

Promoción

Numero del catalogo: (70856-3)

Proveedor: Merck Millipore (Novagen)

Descripción: Convenient solution for selective degradation of RNA.

UOM: 1 * 1 mL

Promoción

Proveedor: Merck Millipore (Novagen)

Descripción: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Proveedor: Merck Millipore (Novagen)

Descripción: Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.