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X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido)

Numero del catalogo: (RC-113)

Proveedor: G-Biosciences

Descripción: X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido)

UOM: 1 * 100 mg

Promoción

N/A Each

X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido), white powder

Proveedor: MP Biomedicals

Descripción: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido), Millipore®

Proveedor: Merck Millipore (Calbiochem‎)

Descripción: X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido), Millipore®

X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido) staining kit

Numero del catalogo: (OZBIGX10003)

Proveedor: OZ BIOSCIENCES

Descripción: X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.

UOM: 1 * 1 KIT

Promoción

N/A Each

X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido) substrate ≥99%, Grado biología molecular

Numero del catalogo: (OZBIXG31000)

Proveedor: OZ BIOSCIENCES

Descripción: X-Gal substrate is metabolised by the b-galactosidase enzyme. The quality and purity of the substrate is essential to obtain high-quality and reliable results.

UOM: 1 * 1 g

Promoción

N/A Each

X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido)

Proveedor: G-Biosciences

Descripción: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

2,2,2-Trifluoro-N-metil-N-(trifluoroacetil)acetamida acylation reagent, for GC derivatisation

Proveedor: MACHEREY-NAGEL

Descripción: This derivitisation reagent is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions. MBTFA also forms very volatile derivatives with carbohydrates.

X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido) ≥98%, white powder para biología molecular

Proveedor: MP Biomedicals

Descripción: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.